Neuroxelin blend
Also called PE-22-28 + Pinealon + N-acetyl Semax + N-acetyl Selank
A research-blend name for a four-ingredient cognitive formulation. Each ingredient has early research, but no human trial of the combination was identified, and the exact specification could not be confirmed.
Overview
What it is
A blend described as PE-22-28, Pinealon, N-acetyl Semax and N-acetyl Selank. N-acetyl forms are chemically different from the unmodified peptides and from acetate salts.
How it works
PE-22-28 blocks the TREK-1 ion channel; Pinealon is studied for oxidative stress in nerve cells; Semax affects BDNF signaling in rats; Selank has been studied for anxiety.
What the research shows
PE-22-28 produced antidepressant-like effects in mice. Pinealon improved learning in a rat developmental-stress model. Semax changed BDNF and its receptor in rats. A 2008 study of 62 people with anxiety disorders reported Selank performed similarly to medazepam. None tested the blend or the N-acetyl forms.
Studied for
- Depression-related behaviorPE-22-28, mice
- Learning under stressPinealon, rats
- BDNF signalingSemax, rats
- AnxietySelank, small human study
Safety and regulatory status
Safety and side effects
No blend-specific side-effect data exist. The FDA has flagged immune-response and impurity concerns for compounded Semax and Selank.
Regulatory status
Not approved by the FDA for any use.
Mixing and dosing reference
Reference information only. Read before using this section.
Neuroxelin (48 mg Vial) Dosage Protocol
- Vial
- 48 mg total blend
- Add diluent
- 3 mL
- Concentration
- 16 mg/mL
- 1 unit (U-100)
- 160 mcg total in 0.01 mL
- Route
- Subcutaneous
- How often
- Once per day (subcutaneous), preferably morning.
- Typical range
- 250–1000 mcg once daily (standard dose: 500 mcg).
- Cycle length
- 8–12 weeks; re-assess every 8 weeks for extension.
Standard / Gradual Approach
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–4 | 500 mcg | 3 units (0.03 mL) |
| Weeks 5–8 | 750 mcg | 4.7 units (0.047 mL) |
Alternative Dosing Tiers
| Tier | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Conservative | 250 mcg | 1.6 units (0.016 mL) |
| Typical | 500 mcg | 3.1 units (0.031 mL) |
| Aggressive | 1000 mcg | 6.3 units (0.063 mL) |
How to mix
- Allow lyophilized vial to reach room temperature before opening.
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake). Label with concentration (16 mg/mL) and date; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Dosing details
- Start: 500 mcg daily for Weeks 1–4; assess tolerance.
- Adjust: Increase to 750–1000 mcg daily from Week 5 if well-tolerated and more aggressive support is desired.
- Frequency: Once per day (subcutaneous), preferably morning.
- Cycle Length: 8–12 weeks; re-assess every 8 weeks for extension.
- Timing: Administer at the same time each morning; rotate injection sites.
Storage
- Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; shelf life depends on product-specific stability data.
- Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles and follow a validated use-by period for the exact formulation.
- Allow vials to reach room temperature before opening to reduce condensation and moisture uptake.
- Do not assume aliquoting or freezing extends the use-by period without stability data for the exact formulation.
Administration technique
- Clean the vial stopper and skin with alcohol; allow to dry completely.
- Pinch a skinfold; insert the needle at 45° into subcutaneous tissue.
- Do not aspirate for subcutaneous injections; inject slowly and steadily over ~5 seconds.
- Rotate sites systematically (abdomen, thighs, upper arms) to avoid tissue irritation.
- Use room-temperature solution and allow alcohol to dry before injecting to minimize discomfort.
- Wait 2 seconds after injection before withdrawing the needle; apply gentle pressure with gauze if needed.
Important notes
- Use new sterile insulin syringes for each injection; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to prevent tissue irritation or lipohypertrophy.
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose, time, and injection site to maintain consistency.
- For doses ≤6 units, use 30- or 50-unit insulin syringes for improved measurement accuracy.
Protocol source: PeptideDosages.com
Common questions
Is Neuroxelin the same as Semax plus Selank?
No. It also contains PE-22-28 and Pinealon, and uses N-acetyl forms.
Is it a proven nootropic?
No human trial of the blend has shown a cognitive benefit.
What should I do about memory problems?
Get evaluated. Medications, depression, thyroid problems and other conditions can affect memory.
Related in Brain and Cognitive Health
Sources and further reading (5)
- Djillani A, et al. Shortened spadin analogs and antidepressant activity. Front Pharmacol, 2017.
- Arutjunyan A, et al. Pinealon protects rat offspring from prenatal hyperhomocysteinemia, 2012.
- Dolotov OV, et al. Semax regulates BDNF and TrkB in rat hippocampus, 2006.
- Zozulia AA, et al. Selank in generalized anxiety disorder and neurasthenia, 2008.
- Source summary: PeptideDosages.com research overview (rewritten for NVO).